Journal: Pharmaceuticals

Article Title: Antibacterial Activity and Mechanisms of Plant Flavonoids against Gram-Negative Bacteria Based on the Antibacterial Statistical Model

doi: 10.3390/ph17030292

Figure Lengend Snippet: Reported plant flavonoids together with their structure types, physicochemical parameters, and inhibitory activities against E. coli ATCC 25922 [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 ].

Article Snippet: Based on this relationship, the minimum inhibitory concentration (MIC) of plant flavonoids against Gram-positive bacteria can be predicted from their lipophilic physicochemical parameter LogP (Log 10 of partition coefficient) or LogD 7.40 (Log 10 of distribution coefficient at pH 7.40), which is calculated from their chemical structures using the software ACD/Labs.

Techniques:

Journal: Nature Communications

Article Title: CAR-T cell therapy targeting surface expression of TYRP1 to treat cutaneous and rare melanoma subtypes

doi: 10.1038/s41467-024-45221-2

Figure Lengend Snippet: a Kinetic of tumor growth or regression over time after treatment with 20D7SL-28ζ CAR-T cells. Tumor size was measured with a caliper. Mean ± SEM is plotted ( n = 10 mice for untreated, n = 11 mice for UTD and 20D7SL-28z). **** p < 0.0005 vs Untreated, #### p < 0.0005 vs UTD, unpaired, two-tailed t test with Holm-Sidak adjustment for multiple comparisons. Retrovirus vector copy number in the tumor ( b ) and the spleen ( c ) of mice treated with 20D7SL-28ζ CAR-T cell therapy at days 5, 13, and 27 after adoptive T-cell transfer. Mean ± SD is plotted ( n = 10 mice). Complete blood counts at days 5, 13, and 27 after adoptive T-cell transfer with 20D7SL-28ζ CAR-T cells. White blood cells ( d ), hemoglobin ( e ), hematocrit ( f ), and Platelets ( g ) are shown. Mean ± SD are plotted, each dot is one mouse ( d – f) Untreated n = 9 for Day 6 and n = 10 for Day 13, UTD n = 8 for Day 6 and n = 10 for Day 13, 20D7SL-28z n = 8 for Day 6, n = 6 for Day 13 and n = 7 for Day 27; ( g ) Untreated n = 8 for Day 6 and n = 9 for Day 13, UTD n = 8 for Day 6 and n = 9 for Day 13, 20D7SL-28z n = 6 for Day 6, n = 2 for Day 13 and n = 6 for Day 27). Serum chemistry at day 13, after adoptive T-cell transfer with 20D7SL-28ζ CAR-T cells. Mice with tumors treated with untransduced T cells and vehicle were used as controls. Untreated mice without tumors and mice without tumors receiving irradiation only were used as additional controls. Alkaline phosphatase ( h ), Alanine aminotransferase ( i ), aspartate aminotransferase ( j ), and total bilirubin ( k ) are shown. Mean ± SD is plotted ( n = 5 mice). Cytokine release in serum at days 5, 13, and 27 after adoptive T-cell transfer with 20D7SL-28ζ CAR-T cells. IL-6 ( l ), IFNγ ( m ), and IL-10 ( n ) are shown. Mean ± SD are plotted, each dot is one mouse ( l – n ) Untreated n = 7 for Day 5 and n = 9 for Day 13, UTD n = 7 for Day 6 and n = 10 for Day 13, 20D7SL-28z n = 7 for Day 6, n = 6 for Day 13 and n = 10 for Day 27). Black dashed line shows the minimum quantifiable levels using the BD CBA Mouse Inflammation Kit (20 pg/mL). o , p Quantitative measures of the average ONL thickness were taken at 0.2 mm intervals in frames (−10 to 10) to the optic nerve. Mean ± SEM is plotted ( n = 4 mice). q Quantitative TYRP1 pixel intensity in the RPE cells from mice at day 13 and 27 after receiving ACT with untransduced T cells or 20D7SL-28ζ CAR-T cells. Mean ± SD is plotted ( n = 4 mice, three sections of the retina per mouse). * p < 0.05, ** p < 0.005, *** p < 0.001, **** p < 0.0005 unpaired, two-tailed t test with Holm-Sidak adjustment for multiple comparisons. Unless otherwise indicated, differences are not statistically significant. Unless otherwise indicated, untransduced T cells and PBS were used as controls. Source data and exact p values are provided as a file.

Article Snippet: Black dashed line shows the minimum quantifiable levels using the BD CBA Mouse Inflammation Kit (20 pg/mL). o , p Quantitative measures of the average ONL thickness were taken at 0.2 mm intervals in frames (−10 to 10) to the optic nerve.

Techniques: Two Tailed Test, Plasmid Preparation, Irradiation

Journal: bioRxiv

Article Title: Human iPSC-derived hypertrophic chondrocytes reveal a mutation-specific unfolded protein response in chondrodysplasias

doi: 10.1101/2020.05.19.103960

Figure Lengend Snippet: (A-C) Histology of growth plate-like structures from tissue collected on day 56 after transplantation into immunodeficient mice. (top) Safranin O staining, (middle) von Kossa staining, (bottom) immunostaining. Scale bars, 200 μm. Similar results were observed in two or more growth plate-like structures for each clone. (D) von Kossa staining of mutant (top) or isogenic control (bottom) pellets transplanted on day 28 of HI and collected after 20 days in vivo . Similar results were obtained in six pellets transplanted into three different mice (two pellets per mouse). Scale bar, 1 mm. (E) Quantification of von Kossa-positive areas. Values are expressed as mean±SEM. Results are calculated as n=3 from three different mice with two pellets each. (n.s., no significant difference by unpaired two-sided t-test).

Article Snippet: This cell suspension was mixed 1:1 with matrigel (BD) and 100 μl were injected subcutaneously into a minimum of six male immunodeficient NOD/ShiJic-scid Jcl (NOD-SCID) mice (CLEA Japan).

Techniques: Transplantation Assay, Staining, Immunostaining, Mutagenesis, In Vivo

Journal: BMC Pediatrics

Article Title: A review of -multidrug-resistant Enterobacteriaceae in a neonatal unit in Johannesburg, South Africa

doi: 10.1186/s12887-019-1709-y

Figure Lengend Snippet: Carbapenem resistant Enterobacteriaceae determinants and susceptibility profiles

Article Snippet: Isolates were characterised as CRE based on carbapenem Etest® (bioMerieux, Marcy-I’Etoile, France) minimum inhibitory concentration (MIC) testing.

Techniques:

Journal: BMC Pediatrics

Article Title: A review of -multidrug-resistant Enterobacteriaceae in a neonatal unit in Johannesburg, South Africa

doi: 10.1186/s12887-019-1709-y

Figure Lengend Snippet: Carbapenem resistance by year

Article Snippet: Isolates were characterised as CRE based on carbapenem Etest® (bioMerieux, Marcy-I’Etoile, France) minimum inhibitory concentration (MIC) testing.

Techniques: